Qtl mapping using magic arabidopsis recombinant inbred lines. The genetic basis of plant architecture in 10 maize. Pdf design and construction of recombinant inbred lines. Qtl analysis of al tolerance in recombinant inbred lines of. Here, we constructed a highdensity linkage map by resequencing the recombinant inbred lines rils derived from a cross between the japonica variety sn265 and indica variety r99. The availability of the recombinant inbreds and the associated data base constitute an efficient means of mapping new molecular markers in maize. Identification of qtls for blast, bacterial blight, and. Recombinant inbred lines derived from potato interspecific. Five qtl1s were further validated using the segregating populations developed from residual heterozygous lines present in the recombinant inbred line populations. For mouse, the lxs panel of recombinant inbred strains williams et al. Mapping isoflavone qtl with main, epistatic and qtl. Recombinant inbred lines derived from cultivars of pea for. Genetic linkage map of a recombinant inbred lines in tomato.
Recombinant inbred lines derived from potato interspecific hybrids shelley jansky, usda and uwmadison david douches michigan state university. Molecular screening of heterorhabditis bacteriophora inbred. Plant architecture attributes, such as plant height, ear height, and internode number, have played an important role in the historical increases in grain yield, lodging resistance, and biomass in maize zea mays l. Nc 84173 is a horticulturallysuperior, multipledisease resistant inbred line that has been used as a parent in production. Parent strains are crossed to create recombinants that are then inbred to isogenicity, resulting in a permanent resource for trait mapping and analysis. The parental lines were previously subjected to molecular screening for polymorphism. A induction of epigenomic perturbation by means of a mutation in genes involved in chromatin control, followed by selfing plants or sibling mating mammals of conditional intercross f2 c. The use of recombinant inbred lines rils for genetic mapping article pdf available in methods in molecular biology 82. Design and construction of recombinant inbred lines. As each ril is an inbred strain, and so can be propagated eternally, a panel of.
Sep 18, 2018 here, we constructed a highdensity linkage map by resequencing the recombinant inbred lines rils derived from a cross between the japonica variety sn265 and indica variety r99. Recombinant inbred lines derived from cultivars of pea for understanding the genetic basis of variation in breeders traits volume 16 special issue carol moreau, maggie knox, lynda turner, tracey rayner, jane thomas, haidee philpott, steve belcher, keith fox, noel ellis, claire domoney. Inheritance of fresh seed dormancy in recombinant inbred. Identification of qtl tgw12 responsible for grain weight in. The methods and software described here are based around 1200 snp genotypes. Intermated recombinant iinbred lines irils in plants liu et al. Genetic dissection of maize plant architecture with an ultra. Recombinant inbred lines rils can serve as powerful tools for genetic mapping.
Recombinant inbred lines for mapping rflp and phenotypic markers in arabidopsis markers in arabidopsis thaliana. Quantitative trait loci associated with micronutrient. Ril populations have been developed and used for numerous studies in many. The polymorphic markers detected in the parental inbred lines were screened across the genomic dna of all the rils for segregating allelic loci. Localization of qtls for seed color using recombinant inbred lines of brassica napus in different environments. A recombinant inbred strain is an organism with chromosomes that incorporate an essentially permanent set of recombination events between chromosomes inherited from two or more inbred strains. In the present investigation 94 recombinant inbred lines from wheat cross aldan resistant and wh542 susceptible, were used. Aug 20, 2004 recombinant inbred lines rils can serve as powerful tools for genetic mapping. Broman kw 2005 the genomes of recombinant inbred lines. Although a handful of isolated recombinant inbred ri mouse strains were developed during the 1940s and 1950s, the importance of these genetic constructs was not appreciated by any scientist prior to dr. Lca is a method of quantifying directional genetic effects i. Recombinant inbred lines rils are a collection of strains that can be used to map quantitative trait loci. Here i describe the process of designing and constructing rils.
Recombinant inbred lines how is recombinant inbred lines. To allow the 4 parental genomes ju1511, ju1941, ju1926, and ju1931 to recombine, we set up a crossing scheme in which 2 pairs of wild isolates were crossed and both the obtained f1 populations were reciprocally intercrossed fig. Genetic analysis of recombinant inbred lines of wheat for. A recombinant inbred line population of tomato and its genetic map 443 derived from a cross between two inbred lines via selfbreeding and singleseed descent breeding approach until homozygosity or near homozygosity is reached 11. Analysis using snpbinner is performed in three parts. Identification of quantitative trait loci for resistance. In this study, sixteen tgw qtl were identified from a recombinant inbred line ril population derived from the cross between the introgression line k1561 of oryza minuta and the rice cultivar g1025. An ril is formed by crossing two inbred strains followed by repeated selfing or sibling mating to create a new inbred line whose genome is a mosaic of the parental genomes figure 1.
Families of recombinant inbred strains numbering from 25 to 5000 are often used to map the locations of dna sequence differences that cont. Our results might help understanding the genetic control of micronutrient concentration and allow the. B104 cross and four inbred line checks b73, mo17, nc300, and b104, scored across four environments tifton, ga, and clayton, nc, in 2004 and. Genetic analysis of recombinant inbred lines for sorghum. Analyzing the genetic basis of variation in plant architecture using high density qtl mapping will be of benefit for the breeding of maize for many traits. In this paper we present a novel approach to quantifying genetic architecture that combines recombinant inbred lines ril with line cross analysis lca. Pdf the use of recombinant inbred lines rils for genetic. Pdf quantitative trait loci controlling agronomic traits in.
A multiparent recombinant inbred line population of c. Additionally, one new plant height qtl, qph3, has been. Donald bailey who first conceptualized their potential utility for linkage analysis while. Group numbers 1, 3, 59, 11, and 12 and roman numerals i, ii, vixii correspond to linkage groups in maps by oliver et al. The genetic basis for autoimmunity in nzb mice has been investigated through analysis of recombinant inbred lines produced by mating nzb mice with two different nonautoimmune strains.
However, nasc no longer maintains adds new markers this map. The use of recombinant inbred lines rils for genetic. Recombinant inbred lines by sibling mating 4 advantages of ri lines each strain is an eternal resource. In this study, a series of recombinant inbred lines rils derived from an indicajaponica cross were planted into four typical ricecultivated areas. Construction of epigenetic recombinant inbred lines epiril. Effects of genetic background and environmental conditions. After 6 generations and about 96% of homozygosity, 60 separate recombinant inbred lines rils were recovered.
Several genes at least six were found to be necessary for the production. Genome mapping of enduse quality traits in a wheat. Gpbd4 were raised in a randomized complete block design with two replications during both seasons summer and kharif of 2010 at the botany garden, department of genetics and plant breeding, uas, dharwad, karnataka. We describe a recombinant inbred line ril population of 161 f5 genotypes for the widest euploid cross that can be made to cultivated sorghum sorghum bicolor using conventional techniques, s. This document describes in detail how to install and run software to map qtls in the panel of 703 arabidopsis magic recombinant inbred lines.
Stalk samples were harvested from 191 recombinant inbred lines rils of b73 an inbred line with lowtointermediate levels of cwcs de811 an inbred line with high levels of cwcs at two locations in 1998 and one in 1999 and assayed for neutral detergent. Broman1 department of biostatistics, johns hopkins university, baltimore, maryland 21205 manuscript received august 20, 2004 accepted for publication november 5, 2004 abstract recombinant inbred lines rils can serve as powerful tools for genetic mapping. The relationships between the environment, texture, and genetic background of the rils were investigated. A total of 99 recombinant inbred lines rils generated from a cross between oriental azumamugi and occidental type kanto nakate gold of barley hordeum vulgare l. Performance prediction of f hybrids between recombinant. There was significant variation among all the recombinant inbred lines for coefficient of infection of karnal bunt neovossia indica.
F1 and f2 generations are produced by intercrossing the inbred strains. Agronomic traits, kernel characteristics, milling related traits, dough mixing strength, and breadmaking properties were evaluated from a population of 9 recombinant inbred lines rils, two parental lines, and three check varieties grown at three minnesota locations in 2006. One of the inbred populations was developed by crossing a hybrid wheat variety with a shw line, and the other was created by crossing the hybrid variety with the progeny line of the shw line. To identify biotic stress resistance factors in both rice lines, two recombinant inbred lines rils were developed and used as mapping populations. Combined analysis of variance of southern leaf blight resistance scores from a population consisting of 3 recombinant inbred maize lines from a nc300. Using crossover breakpoints in recombinant inbred lines to. Shoot and root traits contribute to drought resistance in.
The magic lines and the genotypes are described in. Construction of epigenetic recombinant inbred lines ep openi. Sarclo2 susceptible with a local check gh3684 resistant to striga gesnerioides infection in the glasshouse of sari, tamalenyankpala in 2009. Additionally, one new plant height qtl1, qph3, has been finemapped to a 600kb genomic region where three candidate genes are located. Tgw12, one of most effective qtl was mapped to the region of 204. However, the number of potential crosses grows rapidly as more and more inbred lines are derived, and the. Recombinant inbred lines qtl mapping using inbred strains for the analysis of the linkage of quantitative traits, recombinant lines are useful because of their isogenic nature, because the genetic similarity of individuals allows for the replication of a quantitative trait locus analysis. Reduce individual variation by phenotyping multiple individuals from each strain. These two rice lines were selected as d onors for biotic resistance in breeding programs for improving rice cultivation in rainfed lowland area in ne. In the present work, we have analyzed the qtls that control al tolerance in a set of recombinant inbred lines ri lines of arabidopsis made by a cross between landsberg erecta ler and colombia col by lister and dean 1993. Quantitative genetic analysis of recombinant inbred lines. The genomes of recombinant inbred lines complex trait. Recombinant inbred lines by sibling mating 10 19 equations for sibmating 20. Dec 15, 2002 in the present work, we have analyzed the qtls that control al tolerance in a set of recombinant inbred lines ri lines of arabidopsis made by a cross between landsberg erecta ler and colombia col by lister and dean 1993.
The segregation data for the map as of 2001 can be downloaded as a text file or downloaded as an excel spreadsheet from nasc. Mccouch, s r and doerge, r w 1995 qtl mapping is rice trends genet 11,482487. An inbred line consists of individuals with the same genotype. A recombinant inbred strain or recombinant inbred line is an organism with chromosomes that incorporate an essentially permanent set of recombination events between chromosomes inherited from two or more inbred strains.
817 813 1157 786 816 727 1017 280 572 1386 855 1281 194 623 1031 1115 357 734 418 858 483 439 397 686 600 636 1079 1429 299 292 855 513 1127 1131 482 884 643 262 28 448 871 1000 370 125 267 916