Akta purifier user manual

The proven design has been verified during development and can be userconfigured to meet specific process demands. A simple, yet flexible, control software for akta protein purification systems. System description, system maintenance and troubleshooting are also. Ge pharmacia akta purifier 100 gmi trusted laboratory. Aktapurifier 10 100 a system pumps mixer 2 4 7 3 1 6 5 2 3 4 5 6 7 1 8 fraction collector frac950 conductivity monitor ph detector inline filter uv cond. Akta fplc system operators manual marshall scientific. It allows users to speed up process scaleup and cleaning between product. Our systems can be customized to include almost any configuration of autosamplers, detectors and pumps. All users must read this entire manual to fully understand the safe use of akta. Evaluating the uv response calculate the uv response ratios in the following way.

Air accumulates easier in the flow cell if the flow restrictor doesnt generate enough backpressure, hence check that the flow restrictor generates the following backpressure. Ge akta explorer amersham pharmacia certified fplc system gmi. Manual flowpath b1 or b2 buffervalveb pump a pump b common inlet a2 a1 common inlet b2 b1. Full programming control is available for almost any imaginable purification. This automatic configuration protects the system from damaging events including over pressure. Ge healthcare akta with the ability to purify virtually any biomolecule, akta protein purification systems can handle the simplest and the toughest of challenges. Information about these options can be found in optional configurations user manual which describes the extended functions of ae. Maintenance manually priming buffer inlet lines open draw air out of buffer line go to system control window manual choose. Get familiarized with its use and have its instruction manual handy. Aktapurifier user guide ge healthcare life sciences manualzz. This set up creates a lower flow rate but at a higher pressure required.

Unicorn 7 features an intuitive interface for creating methods by dragging and dropping predefined phases for a wide range of techniques. Tubing kit changes aktapurifier 100 high flow at delivery 0. Aktapurifier 10 cytiva formerly ge healthcare life sciences. If the leakage persists, replace the assembly according to instructions in the pump p960 user manual. Ge akta purifier user manual download ge akta purifier user manual. Aktapurifier upc 10 cytiva, formerly ge healthcare life. Air in the buffers, do degas the buffers before use. Box900, upc900 monitor, p900 pump, cu950 interface equipped with. Akta labscale protein purification systems are designed for purification of biomolecules, providing speed, performance, and flexibility in research and process development. Sections 1 5 contain the necessary information for operating the instrument. Never place the liquids below the pump unless the user manual states that it is. Aktaexplorer am ers ha m bio scie nce am er sham bio ci n a m e r s h a m b io s c ie n c a m e r s h a m b io s c ie box n c e monitor conductivity ph uv detector 3 wavelengths pumps column valves injector sample valve buffers phcell electrode sample holder pump a buffer switching valve outlet valve.

Aktaprime plus user manual 11002644 edition aa 9 1introduction this section is an introduction to aktaprime plus, the documentation included and the content in this manual. Flowpath buffervalvea a1 or a2 flowpath buffervalveb. Please see the aktadesign optimal configuration user manual for the recommended flow rates. Monitor upc900 user manual 18112555 edition ag vii. It also includes relevant guidance for practical handling and maintenance of instrument components. It will help if they get w10 with some good dev support when it comes out. When the user selects a column, the akta explorer instinctively sets the column volume, flow rate and pressure limit parameters into the method template. Data file 11003543 ac process chromatography aktaprocess aktaprocess is an automated liquid chromatography system built for process scaleup and largescale biopharmaceutical manufacturing fig 1. The aim of this tutorial is to demonstrate the use of the ubiquitous akta systems and prepacked hitrap chromatography media for rapid, mediumscale protein purification. Akta laboratoryscale chromatography systems handbook. This high flow rate is necessary when working with.

Belkin f5u220 user manual vvm worked after a soft reset of the device with the sim inserted but only over wifi. Akta chromatography systems have been a staple in protein purification for the past two decades and used throughout the globe. July 4, different optional configurations might be akta purifier 10 manual required, this is a modular fast performance liquid chromatography system used primarily for fast separations and purifications of proteins. For fast protein liquid chromatography of tens of grams of protein, akta pure 150 can be used with flow rates up to 150 mlmin. The protein production laboratories have two machines.

Systems provide more control than manual purification because of the ability to automatically control the flow rate and monitor the progress of the purification as well as to make controlled gradients and automatically collect fractions. Ge akta explorer certified fplc system gmi trusted lab. Our hplc systems such as the agilent 1100 and 1200 series, akta fplcs, and. Aktadesign purification user manual 8 2423 edition ad introduction to this handbook and unicorn 7. The platform covers all major chromatographic and cross flow filtration techniques, from the research laboratory to process development and manufacturing. Aktaexplorer am ers ha m bio scie nce am er sham bio ci n a m e r s h a m b io s c i en c a m e r s h a m b io s c i e n c e akta system training guide explorer 100 10. Data file 11003543 ac process chromatography aktaprocess. It is intended to be operated as an integrated part of an aktadesign chromatography system running unicorn software. Akta avant user manual protein production and purification. Issuu is a digital publishing platform that makes it simple to publish magazines, catalogs, newspapers, books, and more online.

Uv, cond, ph w2 mixer b a buffer valve b v6 buffer valve a v5 multiple sample injection air sensor. This manual describes the operation of the aktafplc system. Mar 29, 2020 akta purifier manual pdf about this manual. Check valve is clogged or damaged, causing leakage. Akta pure 25 purification system is intended for microgramscale protein purifications in tricorn columns up to milligramscale using hitrap and hiscreen columns. Jun 20, 2019 akta purifier manual pdf about this manual. Akta explorer systems include an extensive column library with details of run parameters for each prepacked column. Aug 12, 2019 akta purifier manual pdf about this manual. Fraction collector frac950 user manual 181956 edition ae 7 1 introduction 1.

Opening the unicorn software windows for manual control and programmable features purgingpriming pumps and tubing. Unicorn 7 cytiva, formerly ge healthcare life sciences. Aktafplc optional configurations user manual 18117446 edition aa. For further details see akta avant user manual and akta pure user manual references on page 14. Fplc standard operating procedure akta purifier system page page 1 of 9 fplc standard operating procedure akta system ge note.

The platform covers all major chromatographic and cross flow filtration techniques, from the research laboratory to. The akta purifier 100 is perfect for application spanning a wide range of wavelengths the akta purifier 100 allows for rate flows of up to 100 ml per minute fast and easy start to purification work preprogrammed application protocols and method templates speed and reliability for every run fast optimisation and peak identificationaccurate result comparisons and peakfractionation column. Purge the pump according to the pump p900 user manual. It also contains an overview of the safety instructions. Akta pure 25 new owners intro the exercise below will give a quick demonstration of how easy and intuitive the akta pure 25 will be for you in demonstrating downstream processing to your students. Air accumulates easier in the flow cell if the flow restrictor doesnt generate enough backpressure, hence check that the flow restrictor generates the following back.

Amersham biosciences ab se751 84 uppsala sweden amersham biosciences uk limited amersham place little chalfont. Akta fplc explorer 100 operators manual marshall scientific. Manual akta purifier page layout dialog box scribd. Follow the instructions given in the frac920 user manual. Protein purification laboratory of molecular biology. Always keep the he inlet above the solution or close the valve. Protein purification the aim of this tutorial is to demonstrate the use of the ubiquitous akta systems and prepacked hitrap chromatography media for rapid, mediumscale protein purification. Contents monitor upc900 user manual 18112555 edition ag vii.

Akta avant operating instructions protein production and. The ge akta purifier 10 is a flexible modular fast protein liquid chromatography system. All users must read this entire manual to fully understand the safe use. Apr 19, 2020 akta purifier manual pdf about this manual. Fraction collector frac950 boyce thompson institute. Shutdown procedure for the akta purifier if you are not planning to continue your experiments the following day, proceed as follows. Gloves and protective eyewear should be worn when handling samples and reagents buffers, however it is preferable that the user remove gloves prior to entering commands via the computer keyboard or mouse. Back pressure increase may indicate treatment is overdue. Akta system training guide school of medicine case. If you manually alter the default values of the column, and thereby exceed the.

File containing module user manuals and component instructions for aktafplc equipment pump p920 user manual monitor upc900 user manual. Ensure that the hg lamp position and the filter is selected according to the wave. Akta systems use intelligent unicorn system control software to combine simplicity with power in protein purification. View and download ge akta start maintenance manual online. Amersham biosciences ge fplc system cambridge scientific. We offer an assortment of refurbished and used liquid chromatography solutions ranging from hplc, lcms to fplc. Loosen the lock knob 3 akta avant user manual 29035184 ae. Akta fplc system operators manual marshall scientific by. Saule saulenhalter injektionsventil inv907 box900 inv907 uv900monitor m925 pumpe p900 p901p903 uvdurchflusszelle mischer m925 anausschalter mischkammer zur abfallflasche.